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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/01/2020 |
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Actualizado : |
24/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PESSOA, D.A.N.; LOPES, J.R.G.; SOUZA, E.M.; CAMPOS, É.M.; MEDEIROS, R.M.T.; COOK, D.; LEE, S.T.; RIET-CORREA, F. |
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Afiliación : |
DANIELLE A.N. PESSOA, Graduate Program in Science and Animal Health, Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brazil; JOSÉ RADMACYO G. LOPES, Graduate Program in Science and Animal Health, Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brazil; EMANUEL M. SOUZA, Setor de Ciências Biológicas, Departamento de Bioquímica, Universidade Federal do Paraná (UFPR), Centro Politécnico, Curitiba, Brazil; ÉDIPO M. CAMPOS, Graduate Program in Science and Animal Health, Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brazil; ROSANE MARIA T. MEDEIROS, Graduate Program in Science and Animal Health, Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brazil; DANIEL COOK, Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, United States; STEPHEN T. LEE, Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, United States; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Herbaspirillum seropedicae as a degrading bacterium of monofluoroacetate: effects of its inoculation in goats by ingesting Amorimia septentrionalis and the concentrations of this compound in plants sprayed with the bacterium. [Herbaspirillum seropedicae como bactéria degradadora de monofluoroacetato de sódio: efeitos de sua inoculação em caprinos ingerindo Amorimia septentrionalis e nas concentrações deste composto em plantas pulverizadas com a bactéria.] |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Pesquisa Veterinaria Brasileira, 2019, Volume 39, Issue 10, Pages 802-806. OPEN ACCESS. Doi: 10.1590/1678-5150-PVB-6305 |
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ISSN : |
0100-736X |
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DOI : |
10.1590/1678-5150-PVB-6305 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: May 28, 2019 / Accepted: June 25, 2019 / Published online: December 02, 2019.
This work was funded by the CNPq Universal Project (Process 402140/2016-8). |
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Contenido : |
ABSTRACT.
Herbaspirillum seropedicae is a nitrogen-fixing bacterium capable of using toxic compounds as a source of carbon. Bacteria with this capacity can be used to make animals resistant to plant poisoning containing monofluoroacetate (MFA), such as Amorimia septentrionalis. The aim of this study was to evaluate if H. seropedicae is efficient in the degradation of MFA present in A. septentrionalis and if the inoculation of this bacterium in goats confers protection to A. septentrionalis intoxication. Two experiments were performed: in the first experiment 12 goats were divided into 2 groups. Goats in Group 1 were orally administered a solution containing the H. seropedicae bacterium for 10 days. From day 10 onwards, they received a daily dose of 5g/kg of A. septentrionalis with the bacteriauntil clinical signs of intoxication were observed. Group 2 goats received only the plant at the same dose, also until the observation of clinical signs of intoxication. The amount of MFA found in A. septentrionalis used in the experiment with goats was 1.6±0.058?g/mg. The total plant dose ingested by all goats in Group 1 was 80.83±12.81g/kg (129.33±20.50mg/kg MFA), which were significantly greater (p<0.05) than those of Group 2 goats (39.16±19.08g/kg plant and 62.66±30.53mg/kg MFA). Group 1 goats took an average of 16.16±2.56 days to develop clinical signs of intoxication, significantly longer (p=0.0012) than Group 2 goats (7.83±3.81 days). Two Group 2 goats died on the same day that they developed clinical signs of intoxication. At necropsy of these two animals, no significant changes were observed. In the second experiment, samples of A. septentrionalis were sprayed with a solution containing H. seropedicae. Before and eight days after spraying, the samples were pressed and dried for quantitation of MFA. The amount of MFA present in samples of A. septentrionalis 8 days after spraying with H. seropedicae was significantly lower (p=0.017) than that found prior to spraying. It can be concluded that administration of H. seropedicae in goats is capable of causing greater resistance to A. septentrionalis intoxication, and spraying the plant with this bacterium significantly reduces the amount of MFA in the plant.
© 2019 Colegio Brasileiro de Patologia Animal. All rights reserved.
RESUMO.
Herbaspirillum seropedicae é uma bactéria fixadora de nitrogênio, capaz de utilizar compostos tóxicos como fonte de carbono. Bactérias com essa capacidade podem ser utilizadas para tornar os animais resistentes à intoxicação por plantas que contém monofluoroacetato (MFA), como Amorimia septentrionalis. O objetivo do presente estudo é avaliar se H. seropedicae é eficiente na degradação do MFA presente em A. septentrionalis e se a inoculação dessa bactéria, em caprinos, confere proteção à intoxicação por A. septentrionalis. Foram realizados dois experimentos: no primeiro experimento foram utilizados 12 caprinos, divididos em dois grupos. Os caprinos do Grupo 1 receberam diariamente, oralmente, uma solução contendo a bactéria H. seropedicae durante 10 dias. A partir do décimo dia passaram a receber, diariamente, além da solução com a bactéria 5g/kg de A. septentrionalis até a observação de sinal clínico de intoxicação. Os caprinos do Grupo 2 receberam apenas a planta na mesma dose, também até que a observação de sinais clínicos de intoxicação. A quantidade de MFA encontrada em A. septentrionalis utilizada no experimento com caprinos foi de 1,6± 0,058µg/mg de planta em média. A dose total de planta ingerida por todos os caprinos do Grupo 1 foi de 80,83±12,81g/kg (129,33±20,50mg/kg de MFA), valores significativamente maiores (p<0,05) do que os dos caprinos do Grupo 2 (39,16±19,08g/kg de planta e 62,66± 30,53mg/Kg de MFA). Os caprinos do Grupo 1 demoraram em média 16,16 ±2,56 dias para desenvolver sinais clínicos da intoxicação, período significativamente maior (p=0,0012) que os caprinos do Grupo 2 (7,83±3,81dias). Dois caprinos do Grupo 2 morreram no mesmo dia que desenvolveram sinais clínicos da intoxicação. Na necropsia desses dois animais não foram observadas alterações significativas. No segundo experimento, amostras de A. septentrionalis foram pulverizadas com uma solução contendo a bactéria H. seropedicae. Antes e oito dias após a pulverização, as amostras foram prensadas e secas para posterior quantificação do MFA. A quantidade de MFA presente nas amostras de A. septentrionalis oito dias após a pulverização com H. seropedicae foi significativamente menor (p=0,017) do que a encontrada antes da pulverização. Pode-se concluir que a administração de H. seropedicae em caprinos é capaz de causar uma maior resistência à intoxicação por A. septentrionalis, e a pulverização da planta com esta bactéria reduz significativamente a quantidade de MFA na planta. MenosABSTRACT.
Herbaspirillum seropedicae is a nitrogen-fixing bacterium capable of using toxic compounds as a source of carbon. Bacteria with this capacity can be used to make animals resistant to plant poisoning containing monofluoroacetate (MFA), such as Amorimia septentrionalis. The aim of this study was to evaluate if H. seropedicae is efficient in the degradation of MFA present in A. septentrionalis and if the inoculation of this bacterium in goats confers protection to A. septentrionalis intoxication. Two experiments were performed: in the first experiment 12 goats were divided into 2 groups. Goats in Group 1 were orally administered a solution containing the H. seropedicae bacterium for 10 days. From day 10 onwards, they received a daily dose of 5g/kg of A. septentrionalis with the bacteriauntil clinical signs of intoxication were observed. Group 2 goats received only the plant at the same dose, also until the observation of clinical signs of intoxication. The amount of MFA found in A. septentrionalis used in the experiment with goats was 1.6±0.058?g/mg. The total plant dose ingested by all goats in Group 1 was 80.83±12.81g/kg (129.33±20.50mg/kg MFA), which were significantly greater (p<0.05) than those of Group 2 goats (39.16±19.08g/kg plant and 62.66±30.53mg/kg MFA). Group 1 goats took an average of 16.16±2.56 days to develop clinical signs of intoxication, significantly longer (p=0.0012) than Group 2 goats (7.83±3.81 days). Two Group 2 goats died on the same day that t... Presentar Todo |
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Palabras claves : |
Amorimia septentrionalis; Degrading bacterium; Goats; Herbaspirillum seropedicae; Monofluoroacetate; Plants sprayed; PLATAFORMA SALUD ANIMAL; Poisoning; Toxic plants. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.scielo.br/pdf/pvb/v39n10/1678-5150-pvb-39-10-802.pdf
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Marc : |
LEADER 06512naa a2200349 a 4500
001 1060686
005 2020-01-24
008 2019 bl uuuu u00u1 u #d
022 $a0100-736X
024 7 $a10.1590/1678-5150-PVB-6305$2DOI
100 1 $aPESSOA, D.A.N.
245 $aHerbaspirillum seropedicae as a degrading bacterium of monofluoroacetate$beffects of its inoculation in goats by ingesting Amorimia septentrionalis and the concentrations of this compound in plants sprayed with the bacterium. [Herbaspirillum seropedicae como bactéria degradadora de monofluoroacetato de sódio: efeitos de sua inoculação em caprinos ingerindo Amorimia septentrionalis e nas concentrações deste composto em plantas pulverizadas com a bactéria.]$h[electronic resource]
260 $c2019
500 $aArticle history: Received: May 28, 2019 / Accepted: June 25, 2019 / Published online: December 02, 2019. This work was funded by the CNPq Universal Project (Process 402140/2016-8).
520 $aABSTRACT. Herbaspirillum seropedicae is a nitrogen-fixing bacterium capable of using toxic compounds as a source of carbon. Bacteria with this capacity can be used to make animals resistant to plant poisoning containing monofluoroacetate (MFA), such as Amorimia septentrionalis. The aim of this study was to evaluate if H. seropedicae is efficient in the degradation of MFA present in A. septentrionalis and if the inoculation of this bacterium in goats confers protection to A. septentrionalis intoxication. Two experiments were performed: in the first experiment 12 goats were divided into 2 groups. Goats in Group 1 were orally administered a solution containing the H. seropedicae bacterium for 10 days. From day 10 onwards, they received a daily dose of 5g/kg of A. septentrionalis with the bacteriauntil clinical signs of intoxication were observed. Group 2 goats received only the plant at the same dose, also until the observation of clinical signs of intoxication. The amount of MFA found in A. septentrionalis used in the experiment with goats was 1.6±0.058?g/mg. The total plant dose ingested by all goats in Group 1 was 80.83±12.81g/kg (129.33±20.50mg/kg MFA), which were significantly greater (p<0.05) than those of Group 2 goats (39.16±19.08g/kg plant and 62.66±30.53mg/kg MFA). Group 1 goats took an average of 16.16±2.56 days to develop clinical signs of intoxication, significantly longer (p=0.0012) than Group 2 goats (7.83±3.81 days). Two Group 2 goats died on the same day that they developed clinical signs of intoxication. At necropsy of these two animals, no significant changes were observed. In the second experiment, samples of A. septentrionalis were sprayed with a solution containing H. seropedicae. Before and eight days after spraying, the samples were pressed and dried for quantitation of MFA. The amount of MFA present in samples of A. septentrionalis 8 days after spraying with H. seropedicae was significantly lower (p=0.017) than that found prior to spraying. It can be concluded that administration of H. seropedicae in goats is capable of causing greater resistance to A. septentrionalis intoxication, and spraying the plant with this bacterium significantly reduces the amount of MFA in the plant. © 2019 Colegio Brasileiro de Patologia Animal. All rights reserved. RESUMO. Herbaspirillum seropedicae é uma bactéria fixadora de nitrogênio, capaz de utilizar compostos tóxicos como fonte de carbono. Bactérias com essa capacidade podem ser utilizadas para tornar os animais resistentes à intoxicação por plantas que contém monofluoroacetato (MFA), como Amorimia septentrionalis. O objetivo do presente estudo é avaliar se H. seropedicae é eficiente na degradação do MFA presente em A. septentrionalis e se a inoculação dessa bactéria, em caprinos, confere proteção à intoxicação por A. septentrionalis. Foram realizados dois experimentos: no primeiro experimento foram utilizados 12 caprinos, divididos em dois grupos. Os caprinos do Grupo 1 receberam diariamente, oralmente, uma solução contendo a bactéria H. seropedicae durante 10 dias. A partir do décimo dia passaram a receber, diariamente, além da solução com a bactéria 5g/kg de A. septentrionalis até a observação de sinal clínico de intoxicação. Os caprinos do Grupo 2 receberam apenas a planta na mesma dose, também até que a observação de sinais clínicos de intoxicação. A quantidade de MFA encontrada em A. septentrionalis utilizada no experimento com caprinos foi de 1,6± 0,058µg/mg de planta em média. A dose total de planta ingerida por todos os caprinos do Grupo 1 foi de 80,83±12,81g/kg (129,33±20,50mg/kg de MFA), valores significativamente maiores (p<0,05) do que os dos caprinos do Grupo 2 (39,16±19,08g/kg de planta e 62,66± 30,53mg/Kg de MFA). Os caprinos do Grupo 1 demoraram em média 16,16 ±2,56 dias para desenvolver sinais clínicos da intoxicação, período significativamente maior (p=0,0012) que os caprinos do Grupo 2 (7,83±3,81dias). Dois caprinos do Grupo 2 morreram no mesmo dia que desenvolveram sinais clínicos da intoxicação. Na necropsia desses dois animais não foram observadas alterações significativas. No segundo experimento, amostras de A. septentrionalis foram pulverizadas com uma solução contendo a bactéria H. seropedicae. Antes e oito dias após a pulverização, as amostras foram prensadas e secas para posterior quantificação do MFA. A quantidade de MFA presente nas amostras de A. septentrionalis oito dias após a pulverização com H. seropedicae foi significativamente menor (p=0,017) do que a encontrada antes da pulverização. Pode-se concluir que a administração de H. seropedicae em caprinos é capaz de causar uma maior resistência à intoxicação por A. septentrionalis, e a pulverização da planta com esta bactéria reduz significativamente a quantidade de MFA na planta.
653 $aAmorimia septentrionalis
653 $aDegrading bacterium
653 $aGoats
653 $aHerbaspirillum seropedicae
653 $aMonofluoroacetate
653 $aPlants sprayed
653 $aPLATAFORMA SALUD ANIMAL
653 $aPoisoning
653 $aToxic plants
700 1 $aLOPES, J.R.G.
700 1 $aSOUZA, E.M.
700 1 $aCAMPOS, É.M.
700 1 $aMEDEIROS, R.M.T.
700 1 $aCOOK, D.
700 1 $aLEE, S.T.
700 1 $aRIET-CORREA, F.
773 $tPesquisa Veterinaria Brasileira, 2019, Volume 39, Issue 10, Pages 802-806. OPEN ACCESS. Doi: 10.1590/1678-5150-PVB-6305
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
11/11/2019 |
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Actualizado : |
13/11/2019 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
LÓPEZ VALIENTE, S.; MARESCA, S.; RODRÍGUEZ, A.M.; LONG, N.M.; QUINTANS, G.; PALLADINO, R.A. |
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Afiliación : |
SEBASTIÁN LÓPEZ VALIENTE, Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Cuenca del Salado. Argentina; SEBASTIÁN MARESCA, Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Cuenca del Salado. Argentina.; A.M. RODRÍGUEZ, Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Cuenca del Salado. Argentina; N.M. LONG, Departamento de Ciencias Animales y Veterinarias. Universidad de Clemson, Carolina del Sur, Estados Unidos de América.; GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; R.A. PALLADINO, IIPAAS-CONICET, Universidad Nacional de Lomas de Zamora, Buenos Aires, Argentina. |
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Título : |
Efecto de la restricción proteica en vacas multíparas durante la gestación media y tardía en el crecimiento y fertilidad de la progenie femenina. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
In: QUINTANS, G.; IEWDIUKOW, M. (Ed.). Primer Seminario Técnico de Programación Fetal. Montevideo (UY): INIA, 2019. p. 45-51. |
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Serie : |
(INIA Serie Técnica; 252) |
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ISBN : |
978-9974-38-436-1 |
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ISSN : |
1688-9266 |
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Idioma : |
Español |
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Contenido : |
ABSTRACT
The objective of the present work was to investigate the effects of two levels of crude protein (CP) provided to mature dams during late gestation on subsequent heifer’s growth postweaning and reproductive performance. At 121 d prepartum, 68 multiparous Angus cows were blocked by BW and expected calving date and
randomly assigned to low protein (LP, 6% CP) or high protein (HP, 12% CP) at 12 pens per treatment. Following calving, all cow/calf pairs were housed together until weaning, then female progeny were removed and maintained on pasture as a single group. At puberty (P = 0.01) and pregnancy determination (P = 0.05) the HP heifers were heavier than LP heifers. The LM area was greater at 20 mo of age in HP compared to LP heifers (P = 0.01). Serum IGF-1 concentration was greater in HP heifers compared to LP heifers (P = 0.05). No dam nutrition effects were found on offspring age at puberty (P = 0.98), final pregnancy rate (P = 0.28). Protein supplementation during late gestation does not affect reproductive performance of the offspring heifers but did impact their BW evolution. The use of diet with low amount of protein which the female fetus is exposed in utero can affect her subsequent development. |
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Palabras claves : |
CRECIMIENTO; FETAL PROGRAMMING; GROWTH; PROGRAMACIÓN FETAL; PUBERTAD; PUBERTY. |
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Asunto categoría : |
L53 Fisiología Animal - Reproducción |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13785/1/St-252-p-45-51-Lopez-Valiente.pdf
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Marc : |
LEADER 02182naa a2200289 a 4500
001 1060402
005 2019-11-13
008 2019 bl uuuu u00u1 u #d
020 $a978-9974-38-436-1
022 $a1688-9266
100 1 $aLÓPEZ VALIENTE, S.
245 $aEfecto de la restricción proteica en vacas multíparas durante la gestación media y tardía en el crecimiento y fertilidad de la progenie femenina.$h[electronic resource]
260 $c2019
490 $a(INIA Serie Técnica; 252)
520 $aABSTRACT The objective of the present work was to investigate the effects of two levels of crude protein (CP) provided to mature dams during late gestation on subsequent heifer’s growth postweaning and reproductive performance. At 121 d prepartum, 68 multiparous Angus cows were blocked by BW and expected calving date and randomly assigned to low protein (LP, 6% CP) or high protein (HP, 12% CP) at 12 pens per treatment. Following calving, all cow/calf pairs were housed together until weaning, then female progeny were removed and maintained on pasture as a single group. At puberty (P = 0.01) and pregnancy determination (P = 0.05) the HP heifers were heavier than LP heifers. The LM area was greater at 20 mo of age in HP compared to LP heifers (P = 0.01). Serum IGF-1 concentration was greater in HP heifers compared to LP heifers (P = 0.05). No dam nutrition effects were found on offspring age at puberty (P = 0.98), final pregnancy rate (P = 0.28). Protein supplementation during late gestation does not affect reproductive performance of the offspring heifers but did impact their BW evolution. The use of diet with low amount of protein which the female fetus is exposed in utero can affect her subsequent development.
653 $aCRECIMIENTO
653 $aFETAL PROGRAMMING
653 $aGROWTH
653 $aPROGRAMACIÓN FETAL
653 $aPUBERTAD
653 $aPUBERTY
700 1 $aMARESCA, S.
700 1 $aRODRÍGUEZ, A.M.
700 1 $aLONG, N.M.
700 1 $aQUINTANS, G.
700 1 $aPALLADINO, R.A.
773 $tIn: QUINTANS, G.; IEWDIUKOW, M. (Ed.). Primer Seminario Técnico de Programación Fetal. Montevideo (UY): INIA, 2019. p. 45-51.
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